The Steak is the Limit

The Steak is the Limit

Growth of muscle tissue on a plant-based scaffold marks another milestone in the development of cultivated meat using 3D bioprinting

L-R : PhD student Iris Ianovici and Prof. Shulamit Levenberg

The successful creation of edible muscle fibers by bioprinting a plant-based scaffold and living animal cells is the subject of a new article by Professor Shulamit Levenberg and Ph.D. student Iris Ianovici of the Faculty of Biomedical Engineering at the Technion – Israel Institute of Technology, in collaboration with cultivated meat producers Aleph Farms. Other partners in the research described in the article are Dr. Yedidya Zagury, Dr. Idan Redensky, and Dr. Neta Lavon.

It is estimated that besides the scientific-engineering accomplishment, this technology is likely to enable the robust production of cultivated meat at large scale in the near future.

The development of cultivated meat, i.e. meat that does not involve the raising and slaughtering of animals, is a potential solution for the growing need for meat products following population growth, the environmental damage caused by breeding cattle, and the increasing awareness to animal welfare.

To fulfill the promise of cultivated meat to meet various consumer expectations, there is a need for technologies that allow for the production of whole muscle cuts that are as similar as possible – in terms of taste, smell, and culture – to those slaughtered from animals.

Prof. Shulamit Levenberg, a world leader in tissue engineering, became involved in cultivated meat several years ago after recognizing that her inventions in tissue engineering for medical needs are also relevant for growing cultivated meat. Her research on the subject led to the founding of Aleph Farms, which sponsored the research study now being published. Last year, Aleph Farms presented the first cultivated ribeye steak in history — created in the Levenberg lab — and has since pursued the development of new products. Aleph Farms’ CEO is Didier Toubia, Prof. Levenberg is Chief Scientific Advisor, and Dr. Neta Lavon is the company’s CTO.

The ability to produce a wide variety of cultivated meat products was the primary focus of the present research, which sought to develop the technology for creating thicker cultivated steaks all the while using alternative materials as scaffolds for this purpose

Enabling the perfusion of nutrients across the thicker tissue has been a significant challenge, with most of the currently used scaffolding materials for growing tissues being derived from animals. In the article, the Technion researchers present a solution to these challenges by using an alternative bio-ink to bioprint scaffolds from animal-free proteins, as well as living animal cells. The bio-ink contains the cells that will form the muscle tissue – satellite cells originating from a biopsy taken from livestock, and is formulated by combining alginate (a compound found within the cell walls of brown algae) and proteins isolated from plants – soy or pea proteins. The printing process enables the creation of protein-enriched scaffolds with different geometries. The printing process is based on a method in which the bio-ink is deposited into a suspension bath that supports the materials during printing.

The images show the adhesion and proliferation properties of satellite cells (in red) on 3D bioprinted scaffolds based on predefined geometry, one week after they were printed on the scaffolds. The process was observed with both scaffolds consisting of a combination of alginate and soy protein (top row) and scaffolds composed of alginate and pea protein (bottom row). In both cases, photos at different enlargements were taken (x5 in the left-hand and middle columns; x10 in the right-hand column).

The results: after the scaffolds were printed with the living animal cells, a high cell viability was observed. Furthermore, the cells successfully matured to create muscle fibers as the tissue grew. Since the geometry of the scaffold can be controlled, it is possible to control the introduction of nutrients and the removal of waste from the developing tissue.

“In the engineering process we developed in the lab, we tried to mimic the natural process of tissue formation inside the animal’s body as much as possible,” said Prof. Levenberg. “The cells successfully adhered to the plant-based scaffold, and the growth and differentiation of the cells proved successful as well. Our bio-ink led to a consistent distribution of the cells across the bioprinted scaffold, promoting growth of the cells on top of it. Since we used non-animal-derived materials, like pea protein, which is non-allergenic, our findings promise greater development of the cultivated meat market moving forward.”

Click here for the paper in Biomaterials